In collaboration with Aquarium Pula, we will establish and characterize two primary cell cultures – adductor muscle and female scallop gonads – evaluate cell morphology and proliferation under temperature, pH, and salinity conditions close to the natural ones, and quantify the impact of species-informed supplements. The supplements under test include sterile-filtered hemolymph from young and maturing scallops and phytoplankton extracts; media will be benchmarked in sterilized Adriatic seawater and in commercial growth medium (L-15). In parallel, an aquarium-tank growth monitoring study provides comparative insight to inform media design and a sustainable cell/tissue sourcing concept. Whole-genome sequencing with draft annotation will establish a foundational omics resource for future line development and quality control. Ethical sourcing uses non-invasive biopsy and established hemolymph protocols wherever feasible; Aquarium Pula contributes husbandry expertise and certified animal-care standards. The same primary-culture, media-optimization, and omics workflow can be applied to additional mollusc species such as mussel, oyster, and clam.