Since the early days of bioprocess engineering shear associated protein aggregation was believed to be a real thread for proteins causing a decrease in production yield accompanied by higher costs. Although some research indicated that moderate shear rates do not aggregate proteins, the scientific consensus today is still not aligned. Recent results suggested that elongational forces, very similar to shear, can unfold proteins. Hence, there is a strong demand for a technical solution to describe extraordinary high shear rates and investigate their impact on protein aggregation, to answer this unsolved mystery in bioprocess engineering.
Among global problems, shortage of water resources is considered one of the grand challenges humankind is facing. With growing industries, water consumption and wastewater treatment becomes more problematic. As the large-scale production of biopharmaceuticals increases worldwide, this industry sector has to develop strategies to minimize the environmental footprint as well.
Most of the active components in drugs either are compounds obtained by chemical synthesis or biomolecules produced by microorganisms (often proteins), which are then called biopharmaceuticals. When we talk about biomolecule production it can be separated into two sections: the up- and downstream-process. The main focus of an upstream-process lies on the preparation of the host cells and their fermentation. After a successful production the target protein needs to be separated from impurities. Protein purification – the downstream process – is an important procedure used to produce biomolecules in a highly pure form for the use in human healthcare.
Co-author: Verena Beck
While for scaling up a production process the main goal is to keep the quality and quantity of a product stable, scaling-down is often used for troubleshooting and testing unit operations. At the microscale various process parameters such as temperatures, buffer additives or mixing conditions can be tested much faster and with lower material consumption compared to large scale. Researchers of acib investigated the most crucial parameters affecting the mixing behaviour at the microscale and how mixing of fluids in small scale can be compared to large vessels.