The Development of efficient Enzymatic Cascades in well-coordinated One-Pot-Systems
The HOTZYMES project developed a novel concept to control enzymes through magnetic heating. Enzymes were coupled to magnetic nanoparticles that generated local micro-temperatures under an alternating magnetic field. These enzyme–particle conjugates were integrated into porous microparticles to enable separation, reuse, and application in bioreactors. In addition, a new generation of magnetic bioreactors for biocatalysis was designed and built.
Publications
Continuous production of magnetic iron oxide nanocrystals by oxidative precipitation, Chemical Engineering Journal, Vol 393, 2020, 124593: https://doi.org/10.1016/j.cej.2020.124593
Stabilization of b-Glucuronidase by Immobilization in Magnetic-Silica Hybrid Supports, Catalysts, 2020, Vol 10 (6), p669: https://doi.org/10.3390/catal10060669
Real-Time Intracellular Temperature Imaging Using Lanthanide-Bearing Polymeric Micelles, Nano Lett, 2020, Vol 20 (9), p6466-6472: https://pubs.acs.org/doi/10.1021/acs.nanolett.0c02163
Iron nanoparticle bio-interactions evaluated in Xenopus laevis embryos, a model for studying the safety of ingested nanoparticles, Nanotoxicology, 2020, Vol 14 (2): https://www.tandfonline.com/doi/full/10.1080/17435390.2019.1685695
Magnetic hypothermia with e-Fe2O3 nanoparticles, RSC Adv. 2020, Vol 10, 28786: https://pubs.rsc.org/en/content/articlelanding/2020/RA/D0RA04361C#!divAbstract
Self-sufficient asymmetric reduction of b-ketoesters catalysed by a novel and robust thermophilic alcohol dehydrogenase co-immobilized with NADH, Catalysis Science and Technology, 2021: https://doi.org/10.1039/D1CY00268F
Efficient Brownian oscillators and nanoheaters based on gallium-doped ε-Fe2O3, Chem. Commun., 2021,57, 2285-2288: https://pubs.rsc.org/en/content/articlelanding/2021/cc/d0cc07309a
The Thermodynamics of Medial Vascular Calcification, Front. Cell Dev. Biol. 9:633465 (2021): https://doi.org/10.3389/fcell.2021.633465
Selective Magnetic Nanoheating: Combining Iron Oxide Nanoparticles for Multi-Hot-Spot Induction and Sequential Regulation, Nano Lett. 2021, 21, 17, 7213–7220: https://pubs.acs.org/doi/10.1021/acs.nanolett.1c02178
The Yin and Yang of epigenetics in the field of nanoparticles, Nanoscale Adv., 2022,4, 979-994: https://pubs.rsc.org/en/content/articlelanding/2022/na/d1na00682g
Photonic and magnetic materials for on-demand local drug delivery, https://doi.org/10.1016/j.addr.2022.114584
Mixing iron oxide nanoparticles with different shape and size for tunable magneto-heating performance, Nanoscale, 2021, 13, p5714: https://pubs.rsc.org/en/content/articlelanding/2021/nr/d0nr09121a#!divAbstract
Stabilization of b-Glucuronidase by Immobilization in Magnetic-Silica Hybrid Supports, Catalysts, 2020, Vol 10 (6), p669: https://doi.org/10.3390/catal10060669
Real-Time Intracellular Temperature Imaging Using Lanthanide-Bearing Polymeric Micelles, Nano Lett, 2020, Vol 20 (9), p6466-6472: https://pubs.acs.org/doi/10.1021/acs.nanolett.0c02163
Iron nanoparticle bio-interactions evaluated in Xenopus laevis embryos, a model for studying the safety of ingested nanoparticles, Nanotoxicology, 2020, Vol 14 (2): https://www.tandfonline.com/doi/full/10.1080/17435390.2019.1685695
Magnetic hypothermia with e-Fe2O3 nanoparticles, RSC Adv. 2020, Vol 10, 28786: https://pubs.rsc.org/en/content/articlelanding/2020/RA/D0RA04361C#!divAbstract
Self-sufficient asymmetric reduction of b-ketoesters catalysed by a novel and robust thermophilic alcohol dehydrogenase co-immobilized with NADH, Catalysis Science and Technology, 2021: https://doi.org/10.1039/D1CY00268F
Efficient Brownian oscillators and nanoheaters based on gallium-doped ε-Fe2O3, Chem. Commun., 2021,57, 2285-2288: https://pubs.rsc.org/en/content/articlelanding/2021/cc/d0cc07309a
The Thermodynamics of Medial Vascular Calcification, Front. Cell Dev. Biol. 9:633465 (2021): https://doi.org/10.3389/fcell.2021.633465
Selective Magnetic Nanoheating: Combining Iron Oxide Nanoparticles for Multi-Hot-Spot Induction and Sequential Regulation, Nano Lett. 2021, 21, 17, 7213–7220: https://pubs.acs.org/doi/10.1021/acs.nanolett.1c02178
The Yin and Yang of epigenetics in the field of nanoparticles, Nanoscale Adv., 2022,4, 979-994: https://pubs.rsc.org/en/content/articlelanding/2022/na/d1na00682g
Photonic and magnetic materials for on-demand local drug delivery, https://doi.org/10.1016/j.addr.2022.114584
Mixing iron oxide nanoparticles with different shape and size for tunable magneto-heating performance, Nanoscale, 2021, 13, p5714: https://pubs.rsc.org/en/content/articlelanding/2021/nr/d0nr09121a#!divAbstract
Box Contributions
Magnetic Iron Oxide Colloids for Environmental Applications, Intech Open, 2021: 10.5772/intechopen.95351
Hotzymes Leader Dr. Valeria Grazu explained the project at the European Researchers Night in Graz 2020.
Nicolás Cassinelli, Founder and Director of nB nanoscale Biomagnetics, is talking about the fascinating process of building devices for bioapplications and magnetic heating.
Jesus G. Ovejero is a PostDoc in Madrid, talking about the preparation of magnetic nanoparticles.
Anisha Vyas from Graz, University of Technology explains, how sucrose can be converted to cellobiose with the help of the HOTZYMES technology.
Partners
The research for this work has received funding from the European Union (EU) project HOTZYMES (grant agreement n° 829162) under EU’s Horizon 2020 Programme Research and Innovation actions H2020-FETOPEN-2018-2019-2020-01