High Throughput Screening & Process Development – how small can we get?

Biotech industry has adopted HT screening in micro well plates as process development tools but still the major question remains on how the process can be optimised, so that it is representative of the large scale. Conversely what are the criteria for scaling-up the process developed at the microscale to large scale systems? Meanwhile technologies of even smaller dimensions such as microfluidic devices, lab-on-a chip approaches and HTPD on the nanoscale are discussed as state of the art for process development in the biotech community.

Successful application of HT systems for process development depends on the right choice of technology in combination with Design of experiment strategies or mechanistic modelling and statistical evaluation of the obtained data.

Organizing Comitee:

Astrid Dürauer
Astrid Dürauer
Senior Scientist
BOKU Vienna
Marcel Ottens
Marcel Ottens
Assoc. Professor
TU Delft


Scientific Comitee:

Ana Azvedo, IST; Astrid Dürauer, BOKU Vienna; Karol Lacki, Avitide; Marcel Ottens, TU Delft; Jennifer Pollard, Merck &Co

The symposium will focus on:

  • • Microfluidic devices for up and downstream processing and their limitations
  • • Application of DoE and statistical evaluation of large data
  • • Scale up / down rules for process development in up and downstream processing and their limitations
  • • Nanoscale devices to predict process data
  • • Microscale methods for process development in up and downstream processing – case studies, commercial systems
  • • Lab on a chip approach for process development in up and downstream processing

Questions to be addressed:

  • • How should we generate HT data to get the best out of it?
  • • Are there still open questions to be addressed?
  • • Can we predict production scale processes from microscale experiments?
  • • Which a priori information is necessary to design experiments?
  • • How small is large enough?
Renowned speakers from academia and industry will give keynote lectures on the topic and will be part of a round table discussion. Researchers from academia and industry will be invited for presentations. Participants are invited to submit their work for flash presentations and poster session.

Framework Programme:

A site visit at Boehringer Ingelheim RCV is organized on Tuesday morning (max. 25 participants, first come first serve) including a short introduction of the company and a guided tour to the automation lab at the Process Bioscience Division where a HT platform for process development of E.coli derived products has been set up in collaboration with BOKU Vienna.

Social Programme:

A get together will be organized at a typical Viennese Heurigen.

Who should attend?

  • • PhD students in fields of biotechnology, biochemical engineering, chemicals engineering
  • • All others working in the field of HTPD from industry and academia are highly welcome


View Programme

Wednesday, April 20th, 2022

From 3:00 pmRegistration
4:00 pmWelcome
4:15 pmKeynote I – Lessons learned in building high‐throughput process development capabilities,
Jennifer Pollard, Merck; USA
5:00 pmKeynote II – Design of Experiments and AI: Friends or enemies for mechanistic modelling?
Shuichi Yamamoto, Yamaguchi University, Japan
6 pm to 8 pm Welcome Reception

Thursday, April 21th, 2022

9 am Welcome
Session I: Engineering Fundamentals for Scaling
9:05Can we directly go from microscale to industrial scale – what are the challenges we face?
Astrid Dürauer, BOKU Vienna, Austria
9:30What can we learn from proteins for process development of the new formats?
Alois Jungbauer, BOKU Vienna, Austria
9:55Automated microscale processing: fundamentals and application to biopharmaceuticals production,
Martina Micheletti, University College London, UK
10:20 - 10:40 Coffee Break
Session II: Bioprocess Development
10:45General considerations on scaling and high throughput aspects in Upstream process development,
Gerald Striedner, BOKU Vienna, Austria
11:15Challenges of establishing a fermentation development platform in an industrial environment,
Sandra Abad, Boehringer-Ingelheim RCV, Austria
11:40Moving towards fully autonomous model-based high-throughput bioprocess development and clone discrimination,
Peter Neubauer, TU Berlin, Germany
12:05-1:00 Lunch Break
Session III: Downstream Processing
1:00The real world of preparative protein chromatography: multi-component adsorption,
Rainer Hahn, BOKU Vienna, Austria
1:25Integrated process modelling and machine learning applied to high throughput process development,
Cécile Brocard, Boehringer-Ingelheim RCV, Austria
1:50Scalability of pre-packed preparative chromatography columns with different diameters and lengths - considering extra column effects,
Susanne Schweiger, R&D Croma-Pharma, Austria
2:15 - 2:45 Coffee Break
Session IV: 5`Flash presentations (tba based on the submissions)
2:45 #1
2:50 #2
2:55 #3
3:00 #4
3:05 #5
3:10 #6
3:15 – 4:45 Poster Session
Session V: Keynote and Round Table Discussion
4:45 20 years of HTPD - lessons learned and future directions,
Karol Lacki, Avitide, USA
5:15Round Table Discussion “Quo vadis HTPD – what do we know, what is still missing”
Participants: Cécile Brocard, Karol Lacki, Jennifer Pollard, Shuichi Yamamoto
Moderation: Marcel Ottens, Astrid Dürauer
7 pm Dinner at Heurigen

Friday, April 22th, 2022

Site Visit Boehringer-Ingelheim RCV
9 to 11:30 amSite Visit Boehringer-Ingelheim RCV (max.25 participants, registrations mandatory), Individual travel to and from BI- RCV, Meeting point: 8:45 am Porter ́s Lodge, "Gate Mitte", Belghofergasse 12, 1120 Vienna; Bring your photo identification!
12:00 – 1:00Lunch at BOKU
Topic III: Microfluidics
1:00Nano Scale HTPD,
Marcel Ottens, TU Delft, NL
1:25How to fasten chromatography process development using a microfluidic lab-on-a-chip platform,
Ana Azedvedo, Instituto Superior Técnico, Lisbon, Portugal
1:50From Small Molecules to Cell & Gene Therapy: A Journey in Bioprocess
Nicolas Szita, University College London, UK
2:15Flow Biocatalysis: Challenges and Opportunities for Bioprocessing,
Marco Marques, UCL, University College London, UK
2:40-3:00Coffee Break
Session VII: Big data and Modelling
3:00Statistical Models in Up- and Downstream Processing – their merits, limitations and realization with R,
Michael Melcher, BOKU Vienna, Austria
3:25The beauty of mechanistic models,
Bernt Nilsson, Lund University, Sweden
3:50Advanced machine learning for bioprocess development - How hybrid models can change the perspective,
Mark Dürkop, Novasign, Vienna, Austria
4:15How can computational fluid dynamics help us with scaling challenges? N.N.
4:40Closing Remarks & End of Symposium

Download Programme (PDF)


Includes registration fee, welcome reception, dinner at Heurigen, all coffee and lunch breaks during the symposium. Please send your registration form here.

Early BirdStudent feeRegular fee
Before September 29th, 2021€ 450,–€ 550,–
Late RegistrationStudent feeRegular fee
Until April 30th, 2022€ 500,–€ 550,–

For more information please contact Petra Polak

Abstract Submission:

Abstract for oral or poster presentation can be submitted until December 17th, 2021. Outstanding abstracts will be selected for flash presentations. Use the provided form for your abstract submission. Submit your abstract here.





Speakers (Confirmed):

Sandra Abad
Sandra Abad
Unit Lead Upstream Development at Boehringer Ingelheim RCV, Austria
Ana Azevedo
Ana Azevedo
Ass. Professor at Instituto Superior Técnico, Portugal
Cécile Brocard
Cécile Brocard
Director Downstream Development at Boehringer Ingelheim RCV, Austria
Mark Dürkop
Mark Dürkop
CEO at Novasign GmbH, Austria
Rainer Hahn
Rainer Hahn
Assoc. Professor at BOKU Vienna, Austria
Alois Jungbauer
Alois Jungbauer
Professor at BOKU Vienna, Austria
Karol Lacki
Karol Lacki
Vice President of Technology Development at Avitide, USA
Michael Melcher
Michael Melcher
Scientist at BOKU Vienna, Austria
Marco Marques
Marco Marques
Lecturer at UCL, UK
Martina Micheletti
Martina Micheletti
Senior Scientist UCL, UK
Peter Neubauer
Peter Neubauer
Professor at TU Berlin, Germany
Bernt Nilsson
Bernt Nilsson
Professor at Lund University, Sweden
Jennifer Pollard
Jennifer Pollard
Process Development and Engineering, Merck & Co, USA
Susanne Schweiger
Susanne Schweiger
R&D Croma-Pharma, Austria
Gerald Striedner
Gerald Striedner
Assoc. Professor at BOKU Vienna, Austria
Nicolas Szita
Nicolas Szita
Professor at UCL, UK
Shuichi Yamamoto
Shuichi Yamamoto
Professor at Yamaguchi University, Japan