Cell Design & Engineering
Modern cell design and engineering has to become more rational and integrate aspects of bioprocessing including up- and downstream processing. Predictable cellular performance and high yield production of proteins and biochemicals should become achievable using Systems Biotechnology derived knowledge.
Microorganisms and higher eukaryotic cells are used for biopharmaceutical and technical applications on large scale. However the creation of industrial production strains was mostly based on empirism and screening. Quantitative analysis and modelling (systems biotechnology) of cellular performance and the synthesis, cellular trafficking, and excretion of recombinant proteins and other biomolecules is one of ACIB´s biggest challenges towards rational strain design and engineering.
Biomolecule synthesis and secretion will be analysed by tools of transcriptomics, proteomics, metabolomics and cytometry. Such data provide the basis for dynamic models (e.g. of protein translocation, trafficking, aggregation, degradation and excretion) which can be linked to existing metabolic models. An iterative process of data generation, modelling and cell engineering including new knock down strategies and synthetic biology will advance the efficient predictable generation of future production strains and cell lines.
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Key Researcher |
Prof. Dr. Diethard Mattanovich |
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| Coordination/Contact |
Dr. Concetta Giuliani |
